IFNG and infection: cruzi antibody (yellow), astrocytes cells with anti-GFAP antibody (green), IFNγ+ cells with PE-labelled anti-IFNγ (red) and nuclei revealed by DAPI (blue) showed that frequently the T. cruzi-bearing GFAP+ cells were seen in very close proximity to IFNγ+ cells (Fig. 1A, E) during both acute (67 ± 6.7%) and chronic (55 ± 4.0%) phases of the infection.