Molecular analysis of the hotspots for mutations in eight genes involved in major signaling pathways critically affected in melanoma, employing PCR analysis followed by direct sequencing, disclosed that 15 out of the total 16 cases of female genital tract primary mucosal melanomas displayed wild-type genotypes for exons 2 (codons 12 and 13) and 3 (codon 61) of NRAS, HRAS, and KRAS, for exons 11, 13, 17, and 18 of c-KIT, for exons 9 and 20 of PI3K, and for exons 4 (R183) and 5 (Q209) of GNAQ and GNA11 genes, as shown in Table 2. This evidence concerns the gene HRAS and melanoma.