EEA1 and infection: Importantly, when WLM was inserted into HPV16.L2DM at the original position of FYL (to generate HPV16.L2WLM/DM), a substantial restoration of the L2/TGN46 PLA signal was observed at 16 hours after infection in both cell types, together with a reduction in the EEA1 signal at this time, confirming that the WLM retromer motif restores exit of L2 from the early endosome and trafficking to the Golgi.