For the in vivo analysis, 5 × 105 cells each of the CCR9+ M579-A2 (shControl) and CCR9− M579-A2 (shCCR9) tumor cell lines were subcutaneously implanted in the left and the right flank, respectively, of the NSG immune-deficient mice (scheme in Fig7B). Here, CCR9 is linked to neoplasm.