Using molecular and biochemical assays, we examined the temporal aspects of and mechanistic basis for acetylation and coactivator complex formation in HIF-2 signaling during hypoxia or glucose deprivation for HT1080 cells, a fibrosarcoma-derived cell line in which CBP-mediated acetylation and SIRT1-regulated deacetylation of HIF-2α occurs [14]. Here, EPAS1 is linked to fibrosarcoma.