To test the consistency of the reactivity of mAb 4H84 (detecting denatured HLA-G heavy chain of all HLA-G isoforms) and mAb 5A6G7 (detecting denatured HLA-G5/-G6 heavy chain), 39 cases-matched NSCLC lesions including 13 positive and 26 negative samples detected with mAb 5A6G7, were performed with immunohistochemistry analysis. The gene discussed is HLA-G; the disease is non-small cell lung carcinoma.