To investigate if the limited probe hydrolysis by poor contact between beta-glucuronidase in E. coli and FDGlcU could be reversed by generating soluble beta-glucuronidase in tumors, we i.v. injected E. coli (lux/βG) to allow tumor colonization and then directly injected a mixture of lysozyme/DNase I into tumors to break the bacteria cell wall. The gene discussed is GUSB; the disease is neoplasm.