Moreover, exercise attenuated the expression of proinflammatory cytokines IL-1β and TNF-α and their plasma levels, as well as the expression of inflammatory state and cytokines of the colonic mucosa as well as Hif1α, in rats subjected to HFD, which suggested that moderate exercise selected in our study exerts a beneficial influence on the healing of colonic mucosa in this rodent model of colitis. This evidence concerns the gene TNF and colitis.