These findings are in keeping with an earlier study demonstrating that Rhizoma Atractyloidis acupuncture in HFD-induced obese ICR mice inhibited expression of a number of proteins including C/EBP-α and enhanced the activation of AMPK associated with the inhibition in development of weight gain, hyperglycemia, hyperinsulinemia, and hyperlipidemia as well as the enlargement of fat cell size61. This evidence concerns the gene CEBPA and hyperinsulinism.