EGFR and neoplasm: The aim of the present study was to investigate the impact of the quality of the tissue sample, expressed as a percentage of neoplastic cells in the specimen, as well as the type of analysed lesions, represented by primary or secondary tumours and biopsy (transcutaneous or endoscopic) or surgical specimens, on a sequencing-based mutation analysis of the kinase domain of the EGFR gene in patients with lung adenocarcinomas.