The immunohistofluorescence analyses of PC3 tumor specimens have indicated that 50 mg/kg GDC-0449, or 5 mg/kg docetaxel alone, significantly inhibited the GLI-1 expression in PC3 xenografts as indicated by a decrease of GLI-1-positive red staining relative to untreated PC3 tumors used as control. Here, GLI1 is linked to neoplasm.