Treatment of VEGFR-2-positive LN-308 glioma cells with exogenous VEGF (50 ng/ml) led to increased phosphorylation of VEGFR-2, p90RSK and AKT, confirming active VEGFR-2 signaling in these cells in vitro (Figure S2C). The gene discussed is RPS6KA1; the disease is glioma.