AKT1 and acute lymphoblastic leukemia: However, Jurkat T-ALL cells with a phosphatase and tensin homolog (PTEN) mutation showed constitutive AKT-phosphorylation on both residues T308 and S473 which was inhibited by the dual PI3K/mTOR inhibitor NVP-BEZ235, while no pAKT was detected in the BCP-ALL cell lines (FIGURE 3A).