After 8 minutes of untreated global brain ischemia, at the onset of reperfusion one of three treatment regimens was initiated: intravenous (IV) administration of 0.6 U/kg IGF-1 alone; hypothermia (32 ̊C for 4 h) alone; or combined IGF-1 with hypothermia. This evidence concerns the gene IGF1 and brain ischemia.