Following the administration of NaHS, however, TNF-α, IL-1β and IL-6 levels in the serum and myocardial tissues of rats, as well as ICAM-1 mRNA expression in myocardial tissues and NF-κB protein expression, were decreased, indicating that exogenously supplemented H2S inhibited the synthesis of inflammatory cytokines (such as IL-1β), nuclear transcription factors (such as TNF-α) and adhesion molecules in the serum and myocardial tissues of rats following the development of myocardial ischemia, thereby reducing myocardial injury and protecting myocardial tissues. The gene discussed is TNF; the disease is myocardial ischemia.