In the present study, the effectiveness of a DC-CIK cell co-culture combined with cisplatin chemotherapy in the treatment of cervical cancer was observed, and it was found that the CD3+CD4+ and CD16+CD56+ cell levels in patients following treatment were significantly higher than those prior to treatment, but the proportion of CD4+CD25+ regulatory T cells significantly decreased following treatment (P<0.05), which corresponds well with the results of Yang et al (23). The gene discussed is CD4; the disease is cervical cancer.