In addition, an AML1/ETO-positive Kasumi-1 cell-inoculated AML-M2 xenograft mouse model was established to explore the effects of targeted silencing of HO-1 expression by the green fluorescent protein (GFP)-expressing plasmid pRNAi-siHO-1-GFP on the in vivo proliferation and infiltration of leukemic cells. The gene discussed is RUNX1; the disease is acute myeloid leukemia.