In addition, results from 12 potential off-target sites were negative, indicating that our selected sgRNA is specific to Kcnj13. These findings strongly suggest that loss of Kcnj13 function is postnatal lethal in mice; however, it is interesting to note that a homozygous nonsense mutation (p.Arg166X)17 in KCNJ13 in humans causes LCA disease and not death. This evidence concerns the gene KCNJ13 and Leber congenital amaurosis.