Amplification of the proto-oncogene transcription factor MYCN is found in about 25% of NB cases and correlates with high-risk disease and poor prognosis.20 As SK-N-AS cells are non-MYCN-amplified, the same experiments were repeated with the MYCN-amplified SK-N-BE(2)C cell line. The gene discussed is MYCN; the disease is neuroblastoma.