The mRNA and protein expression levels of P16, Cyclin D1 and pRb in SiHa and SiHa-DDP cell lines were detected, and p16 knock down of a SiHa-DDP cell line was performed to investigate the possible mechanism of DDP chemoresistance, which may lead to the development of novel treatment strategies for chemoresistant cervical carcinoma. Here, RB1 is linked to cervical carcinoma.