To mimic hyperglycemia in diabetes patients, the influence of elevated glucose levels on the actions of the different growth factors was also studied: iBREC were cultivated for 3 days in medium containing 3 g/l (≈17 mM) D-glucose instead of the normal 1 g/l (≈5.6 mM) D-glucose before VEGF-A165 and VEGF-B were added. This evidence concerns the gene VEGFB and diabetes mellitus.