As we and many others have reported, here we used four different double labelling immunofluorescence methods to analyse the changes in TCs in human liver fibrosis, including double labelling for CD34 and PDGFR-α, CD34 and PDGFR-β, CD34 and vimentin, and CD34 and c-kit/CD117. Here, CD34 is linked to Hepatic fibrosis.