To show the role of NASH progression in inducing miR21 and its principle target protein Grhl3, qRTPCR analysis was carried out at different time points and in three experimental models of NASH, namely, the two toxin models of NASH in a background of obesity and insulin resistance (DIO+BDCM and DIO+CCl4), and the dietary model of NASH (MCD diet). The gene discussed is GRHL3; the disease is metabolic dysfunction-associated steatohepatitis.