Results showed that ob/ob (Lep KO) mice that were injected with the toxins for induction of NASH had significantly elevated Grhl3 levels as compared to DIO+BDCM mice that had NASH symptoms at 1w post toxin exposure (Fig. 3B) as shown by western blot analysis and band quantification (3 fold increase) (Fig. 3C) (P<0.05). The gene discussed is GRHL3; the disease is metabolic dysfunction-associated steatohepatitis.