Mature adipocytes were either maintained in insulin sensitive conditions [low glucose (LG)] or shifted to insulin resistant conditions by the classical treatment of high glucose and chronic insulin (HG + INS) to generate hyperglycemia and hyperinsulemia or by treatment with low glucose and the OGA inhibitors PUGNAc (LG + PUGNAc) or GlcNAcstatin (LG + GlcNAcstatin) to more specifically elevate global O-GlcNAc levels. This evidence concerns the gene INS and Hyperglycemia.