Activated T cells supernatant (TCCM), because of containing TNF-α and IFN-γ, is able to involve in the up-regulation of adhesion molecules and co stimulatory molecules on a variety of tumor cells.34 sCD40L or an sCD40L chimeric protein can induce cytokines (i.e., IL-12, IFN-α, and TNF-α) and APC to express immune accessory molecules (i.e., CD80, CD83, and CD86) in a manner similar to that of the membrane-bound form of CD40L on activated T.35-37 For enhancing the maturation of CD11c1 myeloid DC, -g was the most potent cytokine. The gene discussed is TNF; the disease is neoplasm.