With and without EDTA exposure, EP1238Y antibody identified Notch1 protein of ~125 kDa, corresponding to the approximate size of NTM of Notch1 (Figure 1A); D3B8 antibody detected N1-ICD-V1754 of ~110 kDa in NSCLC NCI-H23, NCI-H522 and breast cancer MCF-7 cells after EDTA treatment. The gene discussed is NOTCH1; the disease is breast cancer.