In the current study, we aimed to assess the following: i) the status of BRS-3 in skeletal muscle tissue from patients simultaneously diagnosed with OB/T2D, ii) the effects of [D-Tyr6-β-Ala11,Phe13,Nle14] bombesin6–14 on glucose-related processes in primary cultured myocytes, and iii) the role of BRS-3 and its synthetic agonist, as well as the potential therapeutic application of the synthetic agonist for patients with OB/T2D. This evidence concerns the gene BRS3 and type 2 diabetes mellitus.