Here, we have extended this analysis and we have shown also that apoptosis mediated by neddylation inhibition induced either by MLN4924 or by Nedd8 silencing, in human hepatoma cells, is accompanied by a decrease in OXPHOS, an increase in the glycolytic flux and a higher activity of the PEMT pathway (Suppl. This evidence concerns the gene NEDD8 and hepatocellular carcinoma.