Moreover, in Phb1-KO HCC mice, reduced neddylation levels as a result of its pharmacological inhibition using the small molecule inhibitor MLN4924 was associated with a distinguishable metabolic fingerprint associated with tumor regression and characterized by: a) reduced levels of total SM and variety of DAG, succinate, GTP, malonyl-CoA, glycine; and b) augmented glycolic acid, TAG levels and PEMT flux. The gene discussed is PEMT; the disease is hepatocellular carcinoma.