Additionally, gene expression levels of Nedd8 and NAE1, examined by analysis of published microarrays obtained from an ONCOMINE search (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE14520), were increased in a cohort of 225 HCC human samples versus 200 controls [17] (Fig. 1B). This evidence concerns the gene NEDD8 and hepatocellular carcinoma.