The most frequently methylated gene was p14ARF—methylated in 31.82 % (14/44) of tumor samples, followed by RASSF1A methylated in 27.27 % (12/44) and CDKN2B (p15INK4B) which was methylated in 25.00 % (11/44) of cases. The gene discussed is RASSF1; the disease is neoplasm.