Besides, our results showed that IL-8, which was already recognized by other authors as an early marker of acute renal injury [23, 24] and predictor of unstable angina pectoris acute myocardial infarction [25], could also serve as a marker for differentiation of CRS-1 from other CRS types, which is also consistent with our examination. Here, TWIST1 is linked to congenital rubella syndrome.