Accordingly, we determined the impact of BRAF, PTEN and NRAS mutation on the effect of zoledronic acid treatment on cell viability, clonogenic growth, apoptosis induction, cell migration and activation of the downstream signaling pathway in melanoma cells in vitro and on primary tumor growth and spleen-to-liver colonization in mice models of human melanoma cells in vivo. Here, NRAS is linked to melanoma.