As the above experiments showed that both dengue serum and PAF affected on ZO-1 expressionin a dose dependant manner, which was inhibited by the use of a PAFR blocker, and also the effect of dengue serum of ZO-1 expression was inhibited by a PAFR blocker, we next proceeded to determine the effect of dengue sera on trans-endothelial resistance (TEER). The gene discussed is TJP1; the disease is dengue disease.