IRF3 and glioblastoma: In fact, polyI:C-induced IRF3 phosphorylationlevel was lower in 33°C than 37°C in A549 and T84 cells (S2 Fig.).Virus-mediated IRF3 activation was ambiguous at all three culture temperaturesin T84 cells (Fig. 4C, lowerpanels); more HPeV1 virions were produced in T84 than A549 cells at 33°Cand 37°C (Fig. 4B).We also detected IRF3 activation in another HPeV1-susceptible cell line,DBTRG-5MG glioblastoma cells, which showed a similar IRF3 activation pattern asfor HPeV1-infected A549 cells (Fig.4D).