To further investigate the correlation between Notch1 signaling and the EMT process in breast cancer, an immobilized Jagged1 ligand was used to activate stable shNotch1-transfected cells (First, the stable shNotch1-transfected cells were generated, and then stable shNotch1-transfected cells were incubated with the immobilized Jagged1 ligand for 48 h). Here, NOTCH1 is linked to breast carcinoma.