Irradiated MGAR cells pulsed with MBP(85–99) and/or increasing concentrations of scrambled MBP(85–99), J5 or S18 were incubated with Ob.1A12 (an MS patient derived auto-reactive CD4+ T-cell clone which recognizes MBP(85–99) in the context of HLA-DR2b) and examined for proliferative activity and IL-2 production. The gene discussed is CD4; the disease is myeloid sarcoma.