Recently, Brand et al. performed a bead-based immunoassay to measure HE4 serum levels, and set up the mean value of HE4 at 5.11, 2.07, 3.63 ng/mL for pancreatic cancer, benign pancreatic disease and healthy control individuals, respectively [28]. The gene discussed is WFDC2; the disease is familial pancreatic carcinoma.