Evidence of such recognition was shown by the fact that there was positive ICC for both IFN-γ and IL-2 after coculture in the presence of irradiated Renca-LMP2A, whereas after coculturing in the presence of Renca-WT or Renca-VC, no IFN-γ+ or IL-2+ splenocytes were detected (Fig.3A). Here, IL2 is linked to intrahepatic cholangiocarcinoma.