NFATC1 and urothelial carcinoma: We first examined the expression of NFATc1 in human urothelial carcinoma cell lines, UMUC3, TCCSUP, 647V, and 5637, and an immortalized human normal urothelial cell line, SVHUC, by western blotting (Fig. 1A) and reverse transcription (RT)-polymerase chain reaction (PCR) (Fig. 1B).