Application of nicotine that is an exogenous agonist of nAChR induced osteoblast cell proliferation [6,26], increased gene expression of OC, Col1α1 and ALP in a human osteosarcoma cell line [26] whereas the expression of ALP in a murine osteoblast cell line was reduced [6]. This evidence concerns the gene COL1A1 and osteosarcoma.