Simulated ischemia, however, caused an almost 50% reduction in the number of NeuN-labelled cells compared with controls (n = 9; p = 0.021; Fig. 4A) and a similar decrease in THY-1 mRNA levels (n = 8; p = 0.010; Fig. 4B), indicating a reduction in RGC number. Here, THY1 is linked to ischemia.