Moreover, consistent with the stem cyst phenotypes the expression of wild type GFP-ALIX in the alix1 mutant background rescued the number of egg chambers with 32 cells from 49% to 13% (p < 0.05), whereas neither GFP-ALIX-F198D nor GFP-ALIX-I211D expression in alix1 mutant ovaries could rescue the 32-cell phenotype (40% and 39%, respectively, p < 0.05) (S11C Fig.). The gene discussed is PDCD6IP; the disease is cyst.