Overall average/median staining values of canine malignant tumor tissues were 2317/1824 (TCII), 1913/955 (TCII-R) and 27/23% (Ki-67); range of staining values was 0-6817 (TCII), 3-6897 (TCII-R) and 4-61% (Ki-67). This evidence concerns the gene MKI67 and neoplasm.