To examine whether inhibition of LMP2 and MG132 pretreatment reduces infarction in MCAO model, LMP2-shRNAs and Cont-shRNA were designed and cloned into a lentiviral vector to knockdown stroke-induced LMP2 expression and the effects of LMP2 knockdown on infarct volume were then examined in vivo. This evidence concerns the gene PSMB9 and Stroke.