To investigate the effect of ASO in the spinal cord, Hua et al. [102] infused ASO 10–27 continuously for 7 days in the right lateral ventricle at various doses in adult mild SMA mice (type 3 SMA, Smn+/-/SMN2+/+ with 4 copies of SMN2). This evidence concerns the gene SMN2 and proximal spinal muscular atrophy.