Similarly, internalized Agrin antibody was observed in EEA-1-positive early endosomes from 5 min till the observed 30 min (Supplementary Fig. 2e,f), suggesting that secreted and cell surface Agrin is dynamically endocytosed, consistent with its identification by surface biotinylation and SILAC as a surface-enriched protein in HCC cells. The gene discussed is AGRN; the disease is hepatocellular carcinoma.