These results were confirmed by bisulfite pyro-sequencing such that CpG sites around the transcriptional start site (TSS) of ABCA1 were heavily methylated in A2780 and CP70 ovarian cancer cells, while such methylation was either undetectable or low in IOSE cells or other ovarian cancer cells (Figure 1C). This evidence concerns the gene ABCA1 and ovarian carcinoma.