To examine the long-lasting anti-malaria role of splenic CD11c(+) DCs derived from semi-immune mice, CD11c(+) cells was isolated with the purity >95% (Figure 2A) from the spleens of semi-immune mice three to four months after the third cured infection, as well as from age-matched naïve controls and passively transferred these cells to eight-weeks-old naïve mice. This evidence concerns the gene ITGAX and infection.