To determine the intracellular localization of Oct4 in human cancer cells, we examined Oct4 and pOct-T235 in the U87 and U251 glioblastoma cell lines by immunofluorescence microscopy, using the anti-Oct4 and anti-pOct-T235 employed in the above western blot analysis and in our previous study (25), with 293T and NCCIT cells being the negative and positive controls, respectively. Here, POU5F1 is linked to cancer.