Among the above-mentioned issues, it is important to confirm whether knockdown of YAP using RNAi significantly inhibited liver cancer cell growth; therefore, as YAP was found to be associated with LATS1 in the Hippo pathway, the aim of the present study was to measure the expression of LATS1 in cancer cells in which YAP was downregulated. This evidence concerns the gene YAP1 and liver cancer.