In the present study, the JAK2 gene mutation statuses of 130 patients and 10 controls from the Department of Pathology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine (Nanjing, China) were identified, and the corresponding clinical diagnosis was determined to investigate the value of JAK2 mutational analysis in MPN diagnosis. Here, JAK2 is linked to myeloproliferative neoplasm.